GCSE Biology Required Practical: Enzyme Rates of Reaction - Revision history

NRJC at 14:25, 2 November 2019

2019-11-02T14:25:41Z

NRJC at 10:57, 13 April 2019

2019-04-13T10:57:44Z

NRJC: /* Method */

2019-04-13T10:00:59Z

‎Method

NRJC at 09:40, 11 April 2019

2019-04-11T09:40:01Z

NRJC at 20:41, 21 November 2018

2018-11-21T20:41:04Z

NRJC: Replaced content with "==Key Stage 4== ===Meaning=== Investigate the effect of pH on the rate of reaction for an enzyme."

2018-11-21T20:16:57Z

Replaced content with "==Key Stage 4== ===Meaning=== Investigate the effect of pH on the rate of reaction for an enzyme."

NRJC at 16:19, 21 November 2018

2018-11-21T16:19:40Z

NRJC: Created page with "==Key Stage 4== ===Meaning=== Investigate the effect of pH on the rate of reaction for an enzyme."

2018-11-21T15:54:03Z

Created page with "==Key Stage 4== ===Meaning=== Investigate the effect of pH on the rate of reaction for an enzyme."

New page

==Key Stage 4==
===Meaning===
Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]].

← Older revision		Revision as of 14:25, 2 November 2019
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	#Repeat steps 2 to 7 until all different [[pH]] buffers have been tested.		#Repeat steps 2 to 7 until all different [[pH]] buffers have been tested.
	#Plot a graph with [[pH]] on the x-axis and time taken on the y-axis.		#Plot a graph with [[pH]] on the x-axis and time taken on the y-axis.
		+	===References===
		+	====Edexcel====
		+	:[https://www.amazon.co.uk/gp/product/1292120193/ref=as_li_tl?ie=UTF8&camp=1634&creative=6738&creativeASIN=1292120193&linkCode=as2&tag=nrjc-21&linkId=572df39392fb4200db8391d98ae6314e ''Biology core practicals; pH and enzymes, pages 18-19, GCSE Combined Science, Pearson Edexcel '']

@@ Line 1: / Line 1: @@
 ==Key Stage 4==
 ===Meaning===
 Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]].
@@ Line 9: / Line 10: @@
 *[[Control Variable]]s - The temperature of all solutions. The volume of all solutions.
 ====Method====
-#Place [[Test Tube|test tubes]] containing [[starch]] and a range of of [[pH]] [[Buffer Solution|buffer solutions]], and a container of [[amylase]] [[solution]] in a water bath at a temperature of 25°C and wait until all [[solution]]s reach that [[temperature]].
+#Place [[Test Tube|test tubes]] containing a range of of [[pH]] [[Buffer Solution|buffer solutions]], a container of [[starch]] [[solution]] and a container of [[amylase]] [[solution]] in a water bath at a [[temperature]] of around  30°C and wait until all [[solution]]s reach that [[temperature]].
-#Use a pipette to place one drop of Iodine Solution into each depression in a spotting tile.
+#Use a [[pipette]] to place one drop of [[Iodine Solution]] into each depression in a [[Spotting Tile|spotting tile]].
-#Use a clean pipette to place 2cm<sup>3</sup> of each [[pH]] buffer into seperate depressions of the spotting tile, ensuring the pipette is rinsed each time to prevent contamination.
+#Use a clean [[pipette]] or [[syringe]] to place 2cm<sup>3</sup> of each [[pH]] buffer into a [[Test Tube|test tube]].
-#Use a clean pipette to place 2cm<sup>3</sup> of starch solution into a depression on the spotting tile.
+#Use a clean [[pipette]] or [[syringe]] to place 2cm<sup>3</sup> of starch solution into the same [[Test Tube|test tube]].
-#Time how long it takes for the colour to turn from blue-black to orange.
+#Use a clean [[pipette]] or [[syringe]] to place 1cm<sup>3</sup> of [[amylase]] [[solution]] into the same [[Test Tube|test tube]] and start the [[stopwatch]].
-#Repeat steps 4 and 5 until all [[pH]] buffers have been tested.
+#Use a clean [[pipette]] to remove a drop of the [[solution]] form the [[Test Tube|test tube]] and drop it on the spotting tile every 10 seconds, beginning at 0 seconds.
 #Plot a graph with [[pH]] on the x-axis and time taken on the y-axis.

@@ Line 9: / Line 9: @@
 *[[Control Variable]]s - The temperature of all solutions. The volume of all solutions.
 ====Method====
-#Place test tubes containing amylase solution, starch solution and a range of [[pH]] buffer solutions from [[pH]] 1-13 in a water bath at a temperature of 25°C and wait until all [[solution]]s reach that temperature.
+#Place [[Test Tube|test tubes]] containing [[starch]] and a range of of [[pH]] [[Buffer Solution|buffer solutions]], and a container of [[amylase]] [[solution]] in a water bath at a temperature of 25°C and wait until all [[solution]]s reach that [[temperature]].
 #Use a pipette to place one drop of Iodine Solution into each depression in a spotting tile.
 #Use a clean pipette to place 2cm<sup>3</sup> of each [[pH]] buffer into seperate depressions of the spotting tile, ensuring the pipette is rinsed each time to prevent contamination.

@@ Line 3: / Line 3: @@
 Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]].
-===Method===
+===Experiment Version 1===
 *[[Independent Variable]] - [[pH]] of the buffered [[pH]] [[solution]].
 *[[Dependent Variable]] - Time taken for the colour change to take place.
 *[[Control Variable]]s - The temperature of all solutions. The volume of all solutions.
+====Method====
 #Place test tubes containing amylase solution, starch solution and a range of [[pH]] buffer solutions from [[pH]] 1-13 in a water bath at a temperature of 25°C and wait until all [[solution]]s reach that temperature.
 #Use a pipette to place one drop of Iodine Solution into each depression in a spotting tile.

← Older revision		Revision as of 20:41, 21 November 2018
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	===Meaning===		===Meaning===
	Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]].		Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]].
		+
		+	===Method===
		+	*[[Independent Variable]] - [[pH]] of the buffered [[pH]] [[solution]].
		+	*[[Dependent Variable]] - Time taken for the colour change to take place.
		+	*[[Control Variable]]s - The temperature of all solutions. The volume of all solutions.
		+
		+	#Place test tubes containing amylase solution, starch solution and a range of [[pH]] buffer solutions from [[pH]] 1-13 in a water bath at a temperature of 25°C and wait until all [[solution]]s reach that temperature.
		+	#Use a pipette to place one drop of Iodine Solution into each depression in a spotting tile.
		+	#Use a clean pipette to place 2cm<sup>3</sup> of each [[pH]] buffer into seperate depressions of the spotting tile, ensuring the pipette is rinsed each time to prevent contamination.
		+	#Use a clean pipette to place 2cm<sup>3</sup> of starch solution into a depression on the spotting tile.
		+	#Time how long it takes for the colour to turn from blue-black to orange.
		+	#Repeat steps 4 and 5 until all [[pH]] buffers have been tested.
		+	#Plot a graph with [[pH]] on the x-axis and time taken on the y-axis.

← Older revision		Revision as of 20:16, 21 November 2018
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	===Meaning===		===Meaning===
	Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]].		Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]].
−	~~===Method===~~
−	*[[Dependent Variable]] - The antiseptic or antibiotic used.
−	*[[Independent Variable]] - Observe the growth of colonies.
−	*[[Control Variable]]s - The same quantity of antiseptic and antibiotic. The same spread of [[bacteria]] applied to the growth medium. Incubated for the same length of time. Incubated at the same temperature. Only one type of [[bacteria]] used.
−
−	~~#Agar plates prepared with [[bacteria]] will be supplied.~~
−	~~#Mark the underside of an agar plate into three sections making a dot in the centre of each, labeling them 1,2 and 3 and writing your initials and the date on the agar plate.~~
−	~~#Spray the work space with disinfectant and wash your hands with antibacterial soap.~~
−	~~#Take a small circle of filter paper and soak it into one of the three antiseptics/antibiotics.~~
−	~~#Place the circle onto the dot marked at the centre of a section.~~
−	~~#Repeat steps 4 and 5 ensuring the antiseptics are not mixed in any way.~~
−	~~#Use two pieces of sellotape to attach the lid loosely to the top of the agar plate (to ensure oxygen get in).~~
−	~~#Incubate at 25°C for 48 hours.~~
−	~~#Measure the diameter of the area in which [[bacteria]] did not grow.~~

← Older revision		Revision as of 16:19, 21 November 2018
Line 2:		Line 2:
	===Meaning===		===Meaning===
	Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]].		Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]].
		+	===Method===
		+	*[[Dependent Variable]] - The antiseptic or antibiotic used.
		+	*[[Independent Variable]] - Observe the growth of colonies.
		+	*[[Control Variable]]s - The same quantity of antiseptic and antibiotic. The same spread of [[bacteria]] applied to the growth medium. Incubated for the same length of time. Incubated at the same temperature. Only one type of [[bacteria]] used.
		+
		+	#Agar plates prepared with [[bacteria]] will be supplied.
		+	#Mark the underside of an agar plate into three sections making a dot in the centre of each, labeling them 1,2 and 3 and writing your initials and the date on the agar plate.
		+	#Spray the work space with disinfectant and wash your hands with antibacterial soap.
		+	#Take a small circle of filter paper and soak it into one of the three antiseptics/antibiotics.
		+	#Place the circle onto the dot marked at the centre of a section.
		+	#Repeat steps 4 and 5 ensuring the antiseptics are not mixed in any way.
		+	#Use two pieces of sellotape to attach the lid loosely to the top of the agar plate (to ensure oxygen get in).
		+	#Incubate at 25°C for 48 hours.
		+	#Measure the diameter of the area in which [[bacteria]] did not grow.