Difference between revisions of "Aseptic Technique"
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The following '''techniques''' are used to reduce or prevent contamination: | The following '''techniques''' are used to reduce or prevent contamination: | ||
*Wash hands thoroughly with [[antiseptic]] before the experiment. | *Wash hands thoroughly with [[antiseptic]] before the experiment. | ||
| − | *[[Heating]] the [[Inoculating Loop|inoculating loop]] | + | *[[Heating]] the [[Inoculating Loop|inoculating loop]] red hot to kill any unwanted [[micro-organism]]s. |
*Do not allow the [[Inoculating Loop|inoculating loop]] to touch anything other than the desired [[micro-organism]]s and the [[Culture Medium|culture medium]]. | *Do not allow the [[Inoculating Loop|inoculating loop]] to touch anything other than the desired [[micro-organism]]s and the [[Culture Medium|culture medium]]. | ||
*Keep a lid on the [[Culture Medium|culture medium]] at all times when not adding the desired [[micro-organism]]s. | *Keep a lid on the [[Culture Medium|culture medium]] at all times when not adding the desired [[micro-organism]]s. | ||
| − | *Disinfect all surfaces in the area | + | *[[Disinfectant|Disinfect]] all surfaces in the area. |
*Open the lid only enough to allow the [[Inoculation Loop|inoculation loop]] to touch the surface of the [[Culture Medium|culture medium]]. | *Open the lid only enough to allow the [[Inoculation Loop|inoculation loop]] to touch the surface of the [[Culture Medium|culture medium]]. | ||
*Avoid breathing in the direction of the [[Inoculating Loop|inoculating loop]] and [[Culture Medium|culture medium]]. | *Avoid breathing in the direction of the [[Inoculating Loop|inoculating loop]] and [[Culture Medium|culture medium]]. | ||
*Seal the lid over the [[Culture Medium|culture medium]] with two strips of sellotape. However, do not make it airtight as this will cause the growth of [[anaerobic]] [[micro-organism]]s only. | *Seal the lid over the [[Culture Medium|culture medium]] with two strips of sellotape. However, do not make it airtight as this will cause the growth of [[anaerobic]] [[micro-organism]]s only. | ||
| + | ===References=== | ||
| + | ====Edexcel==== | ||
| + | :[https://www.amazon.co.uk/gp/product/1292120207/ref=as_li_tl?ie=UTF8&camp=1634&creative=6738&creativeASIN=1292120207&linkCode=as2&tag=nrjc-21&linkId=22455ff53961978667722edaa64c0be5 ''Aseptic techniques, page 109, GCSE Biology, Pearson, Edexcel ''] | ||
| + | :[https://www.amazon.co.uk/gp/product/1782948120/ref=as_li_tl?ie=UTF8&camp=1634&creative=6738&creativeASIN=1782948120&linkCode=as2&tag=nrjc-21&linkId=dedef775c6a43dbb0a609441525adac0 ''Aseptic techniques, page 180, GCSE Biology, CGP, Edexcel ''] | ||
| + | :[https://www.amazon.co.uk/gp/product/1782946748/ref=as_li_tl?ie=UTF8&camp=1634&creative=6738&creativeASIN=1782946748&linkCode=as2&tag=nrjc-21&linkId=a4f0348fc37d0ba1bb52d27f8679581f ''Aseptic techniques, page 63, GCSE Biology; The Revision Guide, CGP, Edexcel ''] | ||
| + | |||
| + | ====OCR==== | ||
| + | :[https://www.amazon.co.uk/gp/product/0198359810/ref=as_li_tl?ie=UTF8&camp=1634&creative=6738&creativeASIN=0198359810&linkCode=as2&tag=nrjc-21&linkId=d768d99f1a06f7c12fab40e5aef85a55 ''Aseptic technique, pages 230-231, Gateway GCSE Biology, Oxford, OCR ''] | ||
| + | :[https://www.amazon.co.uk/gp/product/1782945660/ref=as_li_tl?ie=UTF8&camp=1634&creative=6738&creativeASIN=1782945660&linkCode=as2&tag=nrjc-21&linkId=83aa4500ad7759e7f401a1c5ba5df758 ''Aseptic techniques, page 97, Gateway GCSE Biology; The Revision Guide, CGP, OCR ''] | ||
Latest revision as of 17:36, 30 November 2019
Contents
Key Stage 4
Meaning
Aseptic technique is a set of procedures used to make sure a culture medium is not contaminated when attempting to culture micro-organisms.
About Aseptic Technique
The following techniques are used to reduce or prevent contamination:
- Wash hands thoroughly with antiseptic before the experiment.
- Heating the inoculating loop red hot to kill any unwanted micro-organisms.
- Do not allow the inoculating loop to touch anything other than the desired micro-organisms and the culture medium.
- Keep a lid on the culture medium at all times when not adding the desired micro-organisms.
- Disinfect all surfaces in the area.
- Open the lid only enough to allow the inoculation loop to touch the surface of the culture medium.
- Avoid breathing in the direction of the inoculating loop and culture medium.
- Seal the lid over the culture medium with two strips of sellotape. However, do not make it airtight as this will cause the growth of anaerobic micro-organisms only.
References
Edexcel
- Aseptic techniques, page 109, GCSE Biology, Pearson, Edexcel
- Aseptic techniques, page 180, GCSE Biology, CGP, Edexcel
- Aseptic techniques, page 63, GCSE Biology; The Revision Guide, CGP, Edexcel