Difference between revisions of "Aseptic Technique"
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*Do not allow the [[Inoculating Loop|inoculating loop]] to touch anything other than the desired [[micro-organism]]s and the [[Culture Medium|culture medium]]. | *Do not allow the [[Inoculating Loop|inoculating loop]] to touch anything other than the desired [[micro-organism]]s and the [[Culture Medium|culture medium]]. | ||
*Keep a lid on the [[Culture Medium|culture medium]] at all times when not adding the desired [[micro-organism]]s. | *Keep a lid on the [[Culture Medium|culture medium]] at all times when not adding the desired [[micro-organism]]s. | ||
| − | *Disinfect all surfaces in the area | + | *[[Disinfectant|Disinfect]] all surfaces in the area. |
*Open the lid only enough to allow the [[Inoculation Loop|inoculation loop]] to touch the surface of the [[Culture Medium|culture medium]]. | *Open the lid only enough to allow the [[Inoculation Loop|inoculation loop]] to touch the surface of the [[Culture Medium|culture medium]]. | ||
*Avoid breathing in the direction of the [[Inoculating Loop|inoculating loop]] and [[Culture Medium|culture medium]]. | *Avoid breathing in the direction of the [[Inoculating Loop|inoculating loop]] and [[Culture Medium|culture medium]]. | ||
*Seal the lid over the [[Culture Medium|culture medium]] with two strips of sellotape. However, do not make it airtight as this will cause the growth of [[anaerobic]] [[micro-organism]]s only. | *Seal the lid over the [[Culture Medium|culture medium]] with two strips of sellotape. However, do not make it airtight as this will cause the growth of [[anaerobic]] [[micro-organism]]s only. | ||
Revision as of 13:07, 11 November 2018
Key Stage 4
Meaning
Aseptic technique is a set of procedures used to make sure a culture medium is not contaminated when attempting to culture micro-organisms.
About Aseptic Technique
The following techniques are used to reduce or prevent contamination:
- Wash hands thoroughly with antiseptic before the experiment.
- Heating the inoculating loop red hot to kill any unwanted micro-organisms.
- Do not allow the inoculating loop to touch anything other than the desired micro-organisms and the culture medium.
- Keep a lid on the culture medium at all times when not adding the desired micro-organisms.
- Disinfect all surfaces in the area.
- Open the lid only enough to allow the inoculation loop to touch the surface of the culture medium.
- Avoid breathing in the direction of the inoculating loop and culture medium.
- Seal the lid over the culture medium with two strips of sellotape. However, do not make it airtight as this will cause the growth of anaerobic micro-organisms only.