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:1. Cut 6 equal volumes of plant tissue (potato).
:2. Dry the plant tissue with paper towel and [[measure]] the [[mass]] of each piece using an [[Electronic Balance|electronic balance]] or find their [[Volume (Space)|volume]] by [[measuring]] length, width and height with [[ruler]] and multiplying them together.
| style="height:20px; width:200px; text-align:center;" |:3. Use a [[Measuring Cylinder]] to measure 10cm<sup>3</sup> of 1.0M [[sugar]] [[solution]] and pour it into a [[Boiling Tube]].
:4. Repeat step 3 with 8cm<sup>3</sup> of [[sugar]] [[solution]] and 2cm<sup>3</sup> of distilled water to give 0.8M [[sugar]] [[solution]], then 6cm<sup>3</sup> and 4cm<sup>3</sup> of distilled water to give 0.6M [[sugar]] [[solution]], repeating this pattern until there is 10cm<sup>3</sup> of 0.0M [[sugar]] [[solution]].
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|[[File:OsmosisPractical5.png|center|200px]]
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| style="height:20px; width:200px; text-align:center;" |:5. Place one piece of plant tissue into each of the 6 [[Boiling Tube|boiling tubes]] leaving them for 12-24 hours (ensuring it is the same time for all).| style="height:20px; width:200px; text-align:center;" |:6. Remove the pieces of plant tissue, drying them with a paper towel and measure their [[mass]].
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:7. Plot a graph showing the concentration of [[sugar]] [[solution]] on the x-axis and the change in [[mass]] of plant tissue on the y-axis.