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Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]].
===MethodExperiment Version 1=======Variables====
*[[Independent Variable]] - [[pH]] of the buffered [[pH]] [[solution]].
*[[Dependent Variable]] - Time taken for the colour change to take place.
*[[Control Variable]]s - The temperature of all solutions. The volume of all solutions.
====Method====
#Place test tubes containing amylase solution, starch solution and a range of [[pH]] buffer solutions from [[pH]] 1-13 in a water bath at a temperature of 25°C and wait until all [[solution]]s reach that temperature.
#Use a pipette to place one drop of Iodine Solution into each depression in a spotting tile.