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===Method===
: 1. Heat an [[Inoculating Loop|inoculating loop]] over a [[Bunsen Burner|bunsen]] flame until it is red hot. This sterilises the [[Inoculating Loop|inoculating loop]]. (Do not place the [[Inoculating Loop|inoculating loop]] down or breath on it as this may contaminate it.)
: 2. Dip the [[Inoculating Loop|inoculating loop]] into a sample of [[micro-organism]]s.
: 3. Slide the lid of the [[Petri Dish|petri dish]] just enough to create space for the [[Inoculating Loop|inoculating loop]] then wipe the [[Inoculating Loop|inoculating loop]] across the top of the [[Culture Medium|culture medium]]. Then replace the lid as quickly as possible. This helps reduce the chance of contamination.
: 4. Tape the sides of the [[Petri Dish|petri dish]] lid down to prevent contamination of the [[Culture Medium|culture medium]], but leave it loose enough that [[air]] can get in to ensure the [[micro-organism]]s get enough [[Oxygen]]. Otherwise only [[anaerobic]] [[micro-organism]]s will grow.
: 5. Label the [[Petri Dish|petri dish]] clearly and incubate at 25°C. Do not incubate at higher [[temperature]]s as this may lead to [[pathogen]]ic [[micro-organism]]s being '''cultured'''.