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===Meaning===
Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]].
===Method===
*[[Dependent Variable]] - The antiseptic or antibiotic used.
*[[Independent Variable]] - Observe the growth of colonies.
*[[Control Variable]]s - The same quantity of antiseptic and antibiotic. The same spread of [[bacteria]] applied to the growth medium. Incubated for the same length of time. Incubated at the same temperature. Only one type of [[bacteria]] used.
#Agar plates prepared with [[bacteria]] will be supplied.
#Mark the underside of an agar plate into three sections making a dot in the centre of each, labeling them 1,2 and 3 and writing your initials and the date on the agar plate.
#Spray the work space with disinfectant and wash your hands with antibacterial soap.
#Take a small circle of filter paper and soak it into one of the three antiseptics/antibiotics.
#Place the circle onto the dot marked at the centre of a section.
#Repeat steps 4 and 5 ensuring the antiseptics are not mixed in any way.
#Use two pieces of sellotape to attach the lid loosely to the top of the agar plate (to ensure oxygen get in).
#Incubate at 25°C for 48 hours.
#Measure the diameter of the area in which [[bacteria]] did not grow.