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: 3. If lipids are present, a red-stained oil layer will form on the surface of the water.
: 4. If no red-stained oil layer forms, the food sample likely does not contain fats.
{| class="wikitable"
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|[[File:SudanIII.png|center|600px]]
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| style="height:20px; width:600px; text-align:center;" |The mixture shows the SudanIII has turned red in the presence of [[lipid]]s.
|}