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Testing for Lipids

Revision as of 20:43, 17 April 2025 by NRJC (talk | contribs)

Contents

Key Stage 3

Meaning

Lipids can be tested for with different methods.

The Paper Test Method
1. Take a small sample of food.
2. Wipe it on a piece of paper.
3. Hold the paper up to the light.
4. If the paper has turned translucent the food contained lipids.
There are two patches where fat has been asborbed by the paper.
The Alcohol Emulsion Test Method
1. Take a small sample of food.
2. Place the food in ethanol and mix by shaking or stirring.
3. Filter the mixture.
4. Add the clear solution to some water.
5. If lipids are present then a white emulsion will appear.
The mixture on the right shows the presence of lipids.
The Sudan III Test Method
1. Mix a small amount of the food sample with water in a test tube.
2. Add a few drops of Sudan III dye to the test tube and mix.
3. If lipids are present, a red-stained oil layer will form on the surface of the water.
4. If no red-stained oil layer forms, the food sample likely does not contain fats.
The mixture shows the SudanIII has turned red in the presence of lipids.