Open main menu

Changes

Chromatography

952 bytes added, 12:15, 22 January 2019
About Chromatography
*[[Separating Mixtures|Separating]] [[solute]]s from a [[solvent]] in [[solution]]. - [[Evaporation of Solutions|Evaporation (Crystallisation)]] or [[Distillation]].
: [[Chromatography]] relies on different [[chemical]]s experiencing different [[force]]s of [[attraction]] between the [[solvent]] used and the [[chromatography]] paper. The [[solvent]] is referred to as the '[[Mobile Phase|mobile phase]]' and acts to carry the [[chemical]] along as the [[solvent]] moves through the [[chromatography]] paper (mobile because it makes the chemical move). The [[chromatography]] paper is referred to as the '[[Stationary Phase|stationary phase]]' and acts to hold the [[chemical]]s in place (stationary because it stops them from moving).: Each [[chemical]] has a [[Retention Factor|Retention Factor (R<sub>f</sub> number)]] which is a [[ratio]] of how far the [[chemical]] moves along the paper compared to how far the [[solvent]] moves along the paper. The larger this [[Retention Factor]] the greater the [[force]] of [[attraction]] experienced by the [[chemical]] to the paper. The [[chemical]] is 'retained' in place.: These [[Retention Factor|R<sub>f</sub>]] numbers are unique to each [[chemical]] and can be used to [[Separating Mixtures|separate]] and identify the [[chemical]].