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Difference between revisions of "GCSE Biology Required Practical: Enzyme Rates of Reaction"

(Replaced content with "==Key Stage 4== ===Meaning=== Investigate the effect of pH on the rate of reaction for an enzyme.")
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==Key Stage 4==
 
==Key Stage 4==
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{{#ev:youtube|https://www.youtube.com/watch?v=8Yqbu56ImXk}}
 
===Meaning===
 
===Meaning===
 
Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]].
 
Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]].
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===Experiment Version 1===
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====Variables====
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*[[Independent Variable]] - [[pH]] of the buffered [[pH]] [[solution]].
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*[[Dependent Variable]] - Time taken for the colour change to take place.
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*[[Control Variable]]s - The temperature of all solutions. The volume of all solutions.
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====Method====
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#Place [[Test Tube|test tubes]] containing a range of of [[pH]] [[Buffer Solution|buffer solutions]], a container of [[starch]] [[solution]] and a container of [[amylase]] [[solution]] in a water bath at a [[temperature]] of around  30°C and wait until all [[solution]]s reach that [[temperature]].
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#Use a [[pipette]] to place one drop of [[Iodine Solution]] into each depression in a [[Spotting Tile|spotting tile]].
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#Use a clean [[pipette]] or [[syringe]] to place 2cm<sup>3</sup> of each [[pH]] buffer into a [[Test Tube|test tube]].
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#Use a clean [[pipette]] or [[syringe]] to place 2cm<sup>3</sup> of starch solution into the same [[Test Tube|test tube]].
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#Use a clean [[pipette]] or [[syringe]] to place 1cm<sup>3</sup> of [[amylase]] [[solution]] into the same [[Test Tube|test tube]] and start the [[stopwatch]].
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#Use a clean [[pipette]] to remove a drop of the [[solution]] form the [[Test Tube|test tube]] and drop it on the spotting tile every 10 seconds, beginning at 0 seconds.
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#Repeat this until the [[Iodine Solution|iodine solution]] in one of the depressions no longer turns black, but instead remains orange.
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#Repeat steps 2 to 7 until all different [[pH]] buffers have been tested.
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#Plot a graph with [[pH]] on the x-axis and time taken on the y-axis.
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===References===
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====Edexcel====
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:[https://www.amazon.co.uk/gp/product/1292120193/ref=as_li_tl?ie=UTF8&camp=1634&creative=6738&creativeASIN=1292120193&linkCode=as2&tag=nrjc-21&linkId=572df39392fb4200db8391d98ae6314e ''Biology core practicals; pH and enzymes, pages 18-19, GCSE Combined Science, Pearson Edexcel '']

Latest revision as of 14:25, 2 November 2019

Key Stage 4

Meaning

Investigate the effect of pH on the rate of reaction for an enzyme.

Experiment Version 1

Variables

Method

  1. Place test tubes containing a range of of pH buffer solutions, a container of starch solution and a container of amylase solution in a water bath at a temperature of around 30°C and wait until all solutions reach that temperature.
  2. Use a pipette to place one drop of Iodine Solution into each depression in a spotting tile.
  3. Use a clean pipette or syringe to place 2cm3 of each pH buffer into a test tube.
  4. Use a clean pipette or syringe to place 2cm3 of starch solution into the same test tube.
  5. Use a clean pipette or syringe to place 1cm3 of amylase solution into the same test tube and start the stopwatch.
  6. Use a clean pipette to remove a drop of the solution form the test tube and drop it on the spotting tile every 10 seconds, beginning at 0 seconds.
  7. Repeat this until the iodine solution in one of the depressions no longer turns black, but instead remains orange.
  8. Repeat steps 2 to 7 until all different pH buffers have been tested.
  9. Plot a graph with pH on the x-axis and time taken on the y-axis.

References

Edexcel

Biology core practicals; pH and enzymes, pages 18-19, GCSE Combined Science, Pearson Edexcel
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