Difference between revisions of "Light Microscope"
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: 4. Tissue is used to absorb any excess [[dye]]. | : 4. Tissue is used to absorb any excess [[dye]]. | ||
: 5. The glass [[slide]] is placed on the '''microscope''' stage. | : 5. The glass [[slide]] is placed on the '''microscope''' stage. | ||
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+ | ===References=== | ||
+ | ====AQA==== | ||
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+ | :[https://www.amazon.co.uk/gp/product/1471851338/ref=as_li_tl?ie=UTF8&camp=1634&creative=6738&creativeASIN=1471851338&linkCode=as2&tag=nrjc-21&linkId=425855d5890466e47189e1c21b67a1ea ''Light microscopes, page 11, GCSE Biology, Hodder, AQA ''] | ||
+ | :[https://www.amazon.co.uk/gp/product/1471851354/ref=as_li_tl?ie=UTF8&camp=1634&creative=6738&creativeASIN=1471851354&linkCode=as2&tag=nrjc-21&linkId=9012a0d354024419214fb3ad5ac44ba0 ''Light microscopes, page 11, GCSE Combined Science Trilogy 1, Hodder, AQA ''] | ||
+ | :[https://www.amazon.co.uk/gp/product/1782945563/ref=as_li_tl?ie=UTF8&camp=1634&creative=6738&creativeASIN=1782945563&linkCode=as2&tag=nrjc-21&linkId=9a1d023a374038e6072f33c4f3cf808b ''Light microscopes, pages 12, 13, GCSE Biology; The Revision Guide, CGP, AQA ''] | ||
+ | :[https://www.amazon.co.uk/gp/product/1782945598/ref=as_li_tl?ie=UTF8&camp=1634&creative=6738&creativeASIN=1782945598&linkCode=as2&tag=nrjc-21&linkId=ad276ad49df77ab4b40ab4fd0fe10032 ''Light microscopes, pages 12, 13, GCSE Combined Science; The Revision Guide, CGP, AQA ''] | ||
+ | :[https://www.amazon.co.uk/gp/product/1782945954/ref=as_li_tl?ie=UTF8&camp=1634&creative=6738&creativeASIN=1782945954&linkCode=as2&tag=nrjc-21&linkId=100574c08fbbb64318256eb79ed61a76 ''Light microscopes, pages 25, 26, GCSE Biology, CGP, AQA ''] | ||
+ | :[https://www.amazon.co.uk/gp/product/1782946381/ref=as_li_tl?ie=UTF8&camp=1634&creative=6738&creativeASIN=1782946381&linkCode=as2&tag=nrjc-21&linkId=5ec5fc3f6429e30c1d9ab9bca2bccf93 ''Light microscopes, pages 25, 26, GCSE Combined Science Trilogy; Biology, CGP, AQA ''] | ||
+ | :[https://www.amazon.co.uk/gp/product/0198359373/ref=as_li_tl?ie=UTF8&camp=1634&creative=6738&creativeASIN=0198359373&linkCode=as2&tag=nrjc-21&linkId=952a73bbb09d222ecc4b50d200679849 ''Light microscopes, pages 4-5, GCSE Biology; Third Edition, Oxford University Press, AQA ''] |
Revision as of 09:10, 8 November 2019
Contents
Key Stage 2
Meaning
A microscope is piece of equipment used to make small objects appear larger.
About Microscopes
- Microscopes can be used to see things too small for our eyes to see.
- Micro means 'small' and scope means 'small'.
Microscope Images
Key Stage 3
Meaning
A microscope is piece of equipment used to make small objects appear larger.
About Microscopes
- Microscopes use convex lenses to magnify an image.
- The parts of the microscope are shown in the diagram below:
- Eyepiece Lens - This is the lens that you look down.
- Coarse Focussing Wheel - This moves the stage by a large amount to bring the image into focus (make the image clear and not blurry).
- Fine Focussing Wheel - This moves the stage by a small amount to focus the image carefully (make the image clear and not blurry).
- Objective Lens - This is the lens next to the specimen that magnifies the image.
- Stage - This where the sample is placed.
- Light Source - This can be a lamp or a mirror used to shine light through the specimen.
Preparing Specimens
To see an object clearly with a microscope a specimen must be prepared first.
- 1. A small specimen is placed on a glass slide.
- 2. A dye is used to colour the specimen so it can be seen more easily under the microscope.
- 3. A cover slip is placed on the specimen to flatten it.
- 4. Tissue is used to absorb any excess dye.
- 5. The glass slide is placed on the microscope stage.
Key Stage 4
Meaning
A light microscope is a device used to observe small objects using visible light.
About Light Microscopes
- A light microscope cannot be used to view anything smaller than about 0.2 micrometres.
- Light microscopes can be used to view objects as small as bacteria but cannot be used to view viruses.
- Eyepiece Lens - This is the lens that you look down.
- Coarse Focussing Wheel - This moves the stage by a large amount to bring the image into focus (make the image clear and not blurry).
- Fine Focussing Wheel - This moves the stage by a small amount to focus the image carefully (make the image clear and not blurry).
- Objective Lens - This is the lens next to the specimen that magnifies the image.
- Stage - This where the sample is placed.
- Light Source - This can be a lamp or a mirror used to shine light through the specimen.
Magnification Equation
Image Size = Object Size X Magnification
\(Magnification = \frac{ImageSize}{ObjectSize}\)
Preparing Specimens
To see an object clearly with a microscope a specimen must be prepared first.
- 1. A small specimen is placed on a glass slide.
- 2. A dye is used to colour the specimen so it can be seen more easily under the microscope.
- 3. A cover slip is placed on the specimen to flatten it.
- 4. Tissue is used to absorb any excess dye.
- 5. The glass slide is placed on the microscope stage.
References
AQA
- Light microscopes, page 11, GCSE Biology, Hodder, AQA
- Light microscopes, page 11, GCSE Combined Science Trilogy 1, Hodder, AQA
- Light microscopes, pages 12, 13, GCSE Biology; The Revision Guide, CGP, AQA
- Light microscopes, pages 12, 13, GCSE Combined Science; The Revision Guide, CGP, AQA
- Light microscopes, pages 25, 26, GCSE Biology, CGP, AQA
- Light microscopes, pages 25, 26, GCSE Combined Science Trilogy; Biology, CGP, AQA
- Light microscopes, pages 4-5, GCSE Biology; Third Edition, Oxford University Press, AQA