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Light Microscope

Revision as of 16:37, 19 February 2022 by NRJC (talk | contribs) (Meaning)

Key Stage 2

Meaning

A picture of a microscope.

A microscope is piece of equipment used to make small objects appear larger.

About Microscopes

Microscopes can be used to see things too small for our eyes to see.
Micro means 'small' and scope means 'small'.

Microscope Images

Key Stage 3

Meaning

A microscope is a piece of equipment used to make small objects appear larger.

About Microscopes

Microscopes use convex lenses to magnify an image.
The parts of the microscope are shown in the diagram below:
MicroscopeDiagram.png
  • Eyepiece Lens - This is the lens that you look down.
  • Coarse Focussing Wheel - This moves the stage by a large amount to bring the image into focus (make the image clear and not blurry).
  • Fine Focussing Wheel - This moves the stage by a small amount to focus the image carefully (make the image clear and not blurry).
  • Objective Lens - This is the lens next to the specimen that magnifies the image.
  • Stage - This is where the sample is placed.
  • Light Source - This can be a lamp or a mirror used to shine light through the specimen.

Preparing Specimens

To see an object clearly with a microscope a specimen must be prepared first.

1. A small specimen is placed on a glass slide.
2. A dye is used to colour the specimen so it can be seen more easily under the microscope.
3. A cover slip is placed on the specimen to flatten it.
4. Tissue is used to absorb any excess dye.
5. The glass slide is placed on the microscope stage.

Key Stage 4

Meaning

A light microscope is a device used to observe small objects using visible light.

About Light Microscopes

A light microscope cannot be used to view anything smaller than about 0.2 micrometres.
Light microscopes can be used to view objects as small as bacteria but cannot be used to view viruses.
MicroscopeDiagram.png
  • Eyepiece Lens - This is the lens that you look down.
  • Coarse Focussing Wheel - This moves the stage by a large amount to bring the image into focus (make the image clear and not blurry).
  • Fine Focussing Wheel - This moves the stage by a small amount to focus the image carefully (make the image clear and not blurry).
  • Objective Lens - This is the lens next to the specimen that magnifies the image.
  • Stage - This is where the sample is placed.
  • Light Source - This can be a lamp or a mirror used to shine light through the specimen.

Magnification Equation

Image Size = Object Size X Magnification

\(Magnification = \frac{ImageSize}{ObjectSize}\)

Preparing Specimens

To see an object clearly with a microscope a specimen must be prepared first.

1. A small specimen is placed on a glass slide.
2. A dye is used to colour the specimen so it can be seen more easily under the microscope.
3. A cover slip is placed on the specimen to flatten it.
4. Tissue is used to absorb any excess dye.
5. The glass slide is placed on the microscope stage.


References

AQA

Light microscopes, page 11, GCSE Biology, Hodder, AQA
Light microscopes, page 11, GCSE Combined Science Trilogy 1, Hodder, AQA
Light microscopes, pages 12, 13, GCSE Biology; The Revision Guide, CGP, AQA
Light microscopes, pages 12, 13, GCSE Combined Science; The Revision Guide, CGP, AQA
Light microscopes, pages 25, 26, GCSE Biology, CGP, AQA
Light microscopes, pages 25, 26, GCSE Combined Science Trilogy; Biology, CGP, AQA
Light microscopes, pages 4-5, GCSE Biology; Third Edition, Oxford University Press, AQA
Microscopes, pages 12, 13, GCSE Biology; The Revision Guide, CGP, AQA
Microscopes, pages 12, 13, GCSE Combined Science; The Revision Guide, CGP, AQA
Microscopes, pages 25-27, 292, GCSE Combined Science Trilogy; Biology, CGP, AQA
Microscopes, pages 25-27, 372, GCSE Biology, CGP, AQA
Microscopes; observing onion cells, pages 25-27, GCSE Biology, CGP, AQA
Microscopes; observing onion cells, pages 25-27, GCSE Combined Science Trilogy; Biology, CGP, AQA

Edexcel

Light microscopes, pages 28, 29, GCSE Biology, CGP, Edexcel

OCR

Light microscopes, page 12-14, Gateway GCSE Biology; The Revision Guide, CGP, OCR
Light microscopes, pages 11-13, Gateway GCSE Combined Science; The Revision Guide, CGP, OCR
Light microscopy, pages 22-23, 25, Gateway GCSE Biology, Oxford, OCR